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                        HIPP-T009 serum-free medium for lymphocytes

                        Completely serum-free media specially designed to promote the in vitro culture of lymphocytes

                        The founding team of BioEngine started the study of in vitro culture of lymphocytes in the 1990s and had made great scientific achievements in this area. For prospective clinical application, the R&D team of BioEngine initiated the design and development of serum-free media for culturing of lymphocytes in vitro in 2015, and successfully obtained a completely serum-free medium formulation in 2018 which was launched with the name of HIPP-T009 in the same year. In 2020, HIPP-T009 became the first homegrown culture medium product used in cell therapy clinical trials in China.
                        HIPP-T009 is an ideal choice for large-scale culture of lymphocytes. It demonstrates not only excellent performance on cell expansion and functional expression but also ensures high safety and stability of the culture process since no serum or other serum substitutes are needed in the culturing system. As HIPP-T009 contains no exogenous cytokines or activators, corresponding cytokines or activators should be added if necessary.

                        HIPP-T009 serum-free medium for lymphocytes

                        Features

                        Xeno-free, contains no growth factors, synthetic activators, or undefined components

                        Universality and flexibility: Applicable for the culture of T-cell, NK cell, CAR-T, CIK, CAR-NK, hematopoietic stem cells and other types of cell lines (NK-92, NK-92MI, K562, Jurkat, etc.)

                        High performance in cell expansion and high-density culture under completely serum-free condition

                        Advantages

                        The 1st approved Chinese culture medium used in cell therapy clinical trials;

                        No need to add serum, plasma, or serum substitutes, hence it minimizes batch-to-batch variation in the culture process and simplifies the declaration process for IND/NDA applications;

                        Manufactured under the cGMP guidelines and ISO13485 quality system, meet regulatory requirements in different countries;

                        Innovative “2 domestic+1 imported” raw material supplier modal ensures a stable supply chain to face the fluctuations and uncertainty in the raw material market;

                        The comprehensive set of DMR documents for audit.

                        Order Information
                        Basal Medium
                        ApplicationProduct NameCat. No.SizeFormProduct Instruction (pdf)Inquiries /Sample application
                        T-cell, NK cell, CAR-T, CIK, CAR-NK-92, hematopoietic stem cells and other types of cell lines (NK-92, NK-92MI, K562, Jurkat, etc.) HIPP-T009  Lymphocyte Serum-free Medium
                        EXP0103801
                        1 L
                        Liquid (non phenol red)
                        Inquiries /Sample application
                        EXP0101302
                        1 L
                        Liquid (containing phenol red)
                        Performance
                        Cell and Gene Therapy

                        CIK cell culture

                        Without adding any serum, the expansion fold of CIK cells during 14 days of culture in a completely serum-free HIPP-T009 medium was significantly higher than that of global Brand A. Even compared with global Brand A with 5% serum added, CIK cell expansion fold and phenotype (CD3+CD56+) in completely serum-free HIPP-T009 are still higher (see below).

                         

                        NK cell culture(With Feeder)

                        Without adding any serum, the expansion performance of NK cells in the completely serum-free HIPP-T009 for 14 days was significantly higher than that of global Brand A. Even compared with global Brand A with 5% serum added, NK cell expansion fold and phenotype (CD3-CD56+) in completely serum-free HIPP-T009 are still higher (see below).

                         

                        NK-92 cell culture

                        Under the same conditions, when cultured in completely serum-free HIPP-T009 for 36 days, the expansion fold and CD3-CD56+ phenotype of NK-92 cells were higher than those in α-MEM supplemented with 12.5% horse serum and 12.5% fetal bovine serum (see below).

                        Let us contact you

                        We have a professional technical team to provide high quality one-stop cell culture service from process development
                        and optimization, medium formulation design to medium processing and manufacturing for all biopharmaceutical companies.

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                        BioEngine

                        3F&4F, Building 3, Lane 396, Lvzhou Ring Road, Minhang District, Shanghai, PRC

                        (86)21-68582660-2792

                        globalsales@bio-engine.com.cn

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