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                        Xeno series and SF003 serum-free medium for MDCK cells

                        Independently developed serum-free media especially for the production of human flu virus and influenza vaccine with MDCK cells

                        The BioEngine R&D team started the research in techniques for the culture of MDCK cells in 1995. Based on more than 10 years of technology accumulation and an AI-intelligent high-throughput data screening platform, BioEngine has released the Xeno series and SF003 MDCK serum-free media for MDCK cells.
                        Xeno series media are applicable for the rapid adaptation of MDCK cells, support high-density culture, and the high-efficiency proliferation of various flu virus subtypes. The series media have been applied in the CTA of human flu vaccines.
                        SF003 is suitable for the rapid adaptation of MDCK cells, supporting high-density culture, as well as the high-efficiency proliferation of avian influenza and swine influenza viruses, and has been applied in several industrial production projects.

                        Xeno series and SF003 serum-free medium for MDCK cells

                        Features

                        Serum free

                        Animal-derived component-free

                        Protein free

                        Support rapid serum-free suspension adaptation of adherent MDCK cells

                        Support high-efficiency proliferation and high-density culture of MDCK cells

                        Xeno supports high-efficiency proliferation of the human flu virus

                        SF003 supports high-efficiency proliferation of avian influenza and swine influenza viruses

                        Advantages

                        Xeno series MDCK Serum-Free Media

                        ADCF; TSE/BSE statement available on demand;

                        Distinctive culture results proven in numerous studies on human flu virus subtypes;

                        Optional powder media for use in large-scale manufacturing with easy preparation procedures;

                        Powder media capable of a single batch size of 100,000 L;

                        Excellent inter-batch consistency (CPK* > 1.33);

                        Full traceability by EU-certified ISO13485:2016 Quality Management System;

                        Complete documents in support of CTA for easier regulatory submission.

                        SF003 MDCK Serum-Free Media

                        Distinctive culture results proven in numerous studies on avian influenza virus subtypes;

                        Optional powder media for use in large-scale manufacturing with easy preparation procedures;

                        Powder media capable of a single batch size of 100,000 L;

                        Excellent inter-batch consistency (CPK* > 1.33);

                        Full traceability by EU-certified ISO13485:2016 Quality Management System. *CPK is a standard index to state the capability of one process. CPK>1.33: the process is capable and meets specification limits. The higher the CPK, the better.

                        Order Information
                        Basal Medium
                        ApplicationProduct NameCat. No.SizeFormProduct Instruction (pdf)Inquiries /Sample application
                        MDCK cells
                        Support high-efficiency proliferation of the human flu virus
                        Xeno-S001 MDCK Serum-Free Medium
                        EXP0100405
                        1 L Liquid Inquiries /Sample application
                        Xeno-S001S MDCK Serum-Free Medium
                        EXP0100403
                        10 L
                        Powder
                        EXP0100406
                        100 L
                        Powder
                        EXP0100401
                        200 L
                        Powder
                        MDCK cells
                        Support high-efficiency proliferation of avian influenza and swine influenza viruses
                        SF003 MDCK Serum-Free Medium
                        EXP0107203
                        10 L
                        Powder
                        EXP0107202
                        100 L
                        Powder
                        EXP0107201
                        200 L
                        Powder
                        Performance
                        Xeno SF003

                        Cell growth

                        When adherent MDCK cells are directly transferred to the suspension culture system with Xeno media, the cells could rapidly adapt to suspension culture and grow steadily, with a doubling time of 18-24 h; after adaptation, the suspension cells are full in shape and uniform in size, and grow as single scattered cells without cell clustering.

                        Xeno media could support a culture density of up to 2.0 × 107 cells/ml of MDCK cells, about double compared with serum-free media of other brands.

                         

                        Virus production

                        When Xeno-S001S media are used to produce various flu virus subtypes, the HA titer could reach up to 29~212 HAU/25 μl.

                        When Xeno-S001S media can be used to produce various flu virus subtypes, the virus titer could reach up to 28~29 TCID50/ml.

                        Cell growth

                        When adherent MDCK cells are directly transferred to the suspension culture system with SF003 media, the cells could rapidly adapt to suspension culture and grow steadily, with a doubling time of 18-24 h; after adaptation, the suspension cells are full in shape and uniform in size, and grow as single scattered cells without cell clustering.

                        SF003 media could support a culture density of up to 2.0×107 cells/ml of MDCK cells, about double compared with serum-free media of other brands.

                         

                        Virus production

                        When SF003 media are used to produce various flu virus subtypes, the HA titer could reach up to 29~212 HAU/25 μl.

                        When SF003 media can be used to produce various flu virus subtypes, the virus titer could reach up to 27~212 TCID50/ml.

                        When SF003 media can be used to produce various flu virus subtypes, the HI titer could reach up to 27~212 HIU/25 μl.

                        Let us contact you

                        We have a professional technical team to provide high quality one-stop cell culture service from process development
                        and optimization, medium formulation design to medium processing and manufacturing for all biopharmaceutical companies.

                        If you have any question, please click "Quick Message" and leave your message,  we will reply to you as soon as possible.
                        If anything urgent, please call (86)21-68582660-2792.

                        BioEngine

                        3F&4F, Building 3, Lane 396, Lvzhou Ring Road, Minhang District, Shanghai, PRC

                        (86)21-68582660-2792

                        globalsales@bio-engine.com.cn

                        Work hours:9:00-17:00
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