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                        Proli series serum-free medium for PK15 cells

                        A serum-free medium especially developed for PK15 cell culture and efficient amplification of circovirus

                        Proli-S001S is an innovative serum-free medium developed independently by Shanghai BioEngine Sci-Tech Co., Ltd., which is serum-free, protein-free, and animal-derived component-free. It is suitable for PK15 suspension cell culture. It supports efficient acclimation of adherent PK15 cells and high-density growth of suspension cells and provides a high-quality environment for efficient amplification of porcine circovirus. Proli-S001S is your ideal choice for large-scale industrial suspension culture of PK15 cells: the suspension culture system provides you with a significant increase in production efficiency, while the serum-free culture system provides you with maximum process stability. It has been industrially applied in the production of porcine circovirus vaccine projects.

                        Proli series serum-free medium for PK15 cells

                        Features

                        Serum-free

                        Animal-derived component-free

                        Protein-free

                        Support rapid serum-free suspension adaptation of adherent PK15 cells

                        Supporting high-density growth of PK15 cells

                        Supporting PK15 cells for efficient production of circovirus

                        Advantages

                        Full traceability by EU-certified ISO13485:2016 Quality Management System;

                        Excellent inter-batch consistency (CPK* > 1.33);

                        Optional powder media for use in large-scale manufacturing with easy preparation procedures;

                        Powder media capable of a single batch size of 100,000 L. *CPK is a standard index to state the capability of one process. CPK>1.33: the process is capable and meets specification limits. The higher the CPK, the better.

                        Order Information
                        Basal Medium
                        Application
                        Product Name
                        Cat. No.
                        Size
                        Form
                        Product Instruction (pdf)Inquiries /Sample application

                        PK15 cells

                        Suitable for production of porcine circovirus

                        Proli-S001 PK15 Serum-free Medium
                        EXP0101001
                        1 L Liquid Inquiries /Sample application
                        Proli-S001S PK15 Serum-free Medium
                        EXP0111103
                        10 L Powder
                        EXP0111102 100 L Powder
                        EXP0111101 200 L Powder
                        Performance
                        Vaccines

                        Cell adaptation

                        Adherent PK15 cells were directly acclimated in Proli-S001S and got stabilized after 10 generations, and the specific growth rate gradually increased with the number of passages, with a doubling time of 22-25h after stabilization.

                        Cell growth

                        Proli-S001S supports high density growth of suspension PK15 cells up to 107 cells/ml.

                        Virus production

                        Using Proli-S001S for culture and suspension of PK15, either for (a) fluorescence quantitative PCR or (b) TCID50 assay showed substantially higher titers of virus production compared to the original adherent process.

                        Let us contact you

                        We have a professional technical team to provide high quality one-stop cell culture service from process development
                        and optimization, medium formulation design to medium processing and manufacturing for all biopharmaceutical companies.

                        If you have any question, please click "Quick Message" and leave your message,  we will reply to you as soon as possible.
                        If anything urgent, please call (86)21-68582660-2792.

                        BioEngine

                        3F&4F, Building 3, Lane 396, Lvzhou Ring Road, Minhang District, Shanghai, PRC

                        (86)21-68582660-2792

                        globalsales@bio-engine.com.cn

                        Work hours:9:00-17:00
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